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Cell Culture

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Cells, Media, Reagents and Supplies (30) Plant Tissue Culture (8) Reference Laboratories (2)

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	Worthington Tissue Dissociation Guide - http://www.tissuedissociation.com/ Resource on cell isolation theory and techniques, including use-tested systems, FAQ, and annotated references searchable by multiple criteria from Lakewood, NJ.
	Bart's Cookbook and Lab Protocols - http://pingu.salk.edu/~sefton/Hyper_protocols/TableOfContentsTC.html Introduction to the steps and reagents involved in tissue culture, at the Sefton Lab, Salk Institute, San Diego, California.
	Plant Tissue Culture - http://www.accessexcellence.org/LC/ST/st2bgplant.html Laboratory protocol for micropropagation from stem tip, node, meristem, embryo or seed. Includes forum maintained by the National Health Museum, Washington, DC.
	Cell Culture Chat - http://www.cellculturechat.com/ Bulletin board for the discussion of cell line and methodology, maintained by an instrument distributor Spectrum Laboratories, Inc., Rancho Dominguez, California.
	Doubling Time - http://www.doubling-time.com/ Downloadable software to compute the doubling time of a cell line, and curve fitting to estimate cell concentration as function of time. [English/Chinese]

Tissue Culture Methods - http://www.research.umbc.edu/~jwolf/method5.htm Describes the equipment and work space requirements for cell and tissue growth. Includes details on cryopreservation, maintenance, media prep procedures, safety considerations and link to the masters degree at University of Maryland Baltimore County.

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